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  • INTERFEROMETRY

    Pall ForteBio site [(http://www.fortebio.com/octet-specs.html). ] The OCTET RED 96 device is a practical alternative to surface plasmon resonance devices. Based on bio-layer interferometry, it is useful for our quantitative measurements. By using dedicated biosensors (8 in a row), it provides kinetics rate constants (Kon, Koff) useful to characterize protein/protein and Ag/Ab interactions, in 96-well (...)

  • BRET and HTRF

    Bioluminescence Resonance energy transfer) et HTRF (Homogenous Time-Resolved Fluore

    The BIOS group is using a Mithras 2 LB243 multimode plate reader equipped with a monochromateur and appropriate filters, for BRET, BRET2, FRET and HTRF data, in populations of living cells.We also use a Tristar 2 LB942 S. By these means, we measure β-arrestin recruitment to GPCRs, G protein trimer dissociation, GPCR dimerization and internalization, second messenger production, antibody/ receptor recognition, etc. Principle of the BRET-based G protein assay (upper image). G protein (...)

  • RPPA

    Reverse-Phase Protein Array

    In 2009, we developed in-house reverse-phase protein arrays (RPPA), using highly sensitive infrared detection. This technology allows the detection of phosphorylated proteins in the sub-attomolar range, from minute amounts of biological material. A phosphorylated protein of interest can then be quantified on-slide, from multiple experimental conditions, paving the way for high-throughput quantification of gonadotropin-induced signalling pathways. Dupuy et al., Proteomics (2009) 9, (...)

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